The Nassib crossing opened an 8 a.m. (0500 GMT), a border official on the Jordanian side in Jaber told Reuters but said there had been no traffic so far. One civilian could be seen waiting to cross.
Jordanian state news agency Petra reported early on Monday that Jordan and Syria had agreed to open the border to all in a change from an earlier statement that normal traffic would not be allowed until a later date.
The Syrian government retook the area around the Nassib border crossing with Jordan in July during a Russian-backed offensive to drive miltiants from their stronghold in southwest Syria.
The closure of the Nassib crossing in 2015 cut a crucial transit route for hundreds of trucks a day transporting goods between Turkey and the Persian Gulf, and Lebanon and the Persian Gulf, in multi-billion dollar annual trade.
Since then, Syria’s only normally operating frontier crossing has been with Lebanon, which itself has no other functioning land borders.
Opening the border will also be important for Lebanon, which relies on Syria for overland connections to all other countries because its only other frontier is with occupied Palestine, with which it has no ties.
Syrian state television footage showed the Syrian side of the crossing as being virtually empty, with just a couple of vehicles present. State news agency SANA said the logistical arrangements for opening the crossing had been completed.
“A Jordanian citizen may leave for Syria in their own car or as a regular passenger. Jordanian cargo is also allowed to leave for Syria according to the Jordanian-Syrian agreement,” Petra said on Monday.
Petra said Syrian coming into Jordan must have obtained prior security clearance from Jordanian authorities to enter the country.
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