"Mr. Trump, Jerusalem is a red line for Muslims," Recep Tayyip Erdogan told a parliamentary group meeting of his ruling Justice and Development (AK) Party, reported Anadolu.
"We are imploring the US once again. You cannot take this kind of step."
Erdogan also warned that if the US does so, Turkey could cut off diplomatic ties with Israeli regime.
"If you take this kind of step, we will convene an Islamic cooperation summit in Istanbul," he added.
The US media has said Trump is considering relocating the American Embassy from Tel Aviv to Jerusalem and the formal recognition of the city as Israeli regime's capital.